Myrna 1.2.0
AWS ID
*
AWS Secret Key
*
AWS Keypair Name
Look it up
Check credentials...
Job name
Job type
Myrna
Just preprocess reads
Input URL
*
Check that input URL exists...
Output URL
*
Check that output URL doesn't exist...
Input type
Preprocessed reads
Manifest file
Truncate length
 (If blank or 0, truncation is disabled)
Skip reads shorter than truncate length
Discard fraction
Quality encoding
Phred+33
Phred+64
Solexa+64
Genes to report in detail
 (If blank or 0, all alignments are discarded)
Genome/Annotation
Human (Ensembl 67)
Mouse (Ensembl 67)
Rat (Ensembl 67)
Chimp (Ensembl 67)
Macaque (Ensembl 67)
Dog (Ensembl 67)
Chicken (Ensembl 67)
Zebrafish (Ensembl 67)
Worm (Ensembl 67)
Fly (Ensembl 67)
Yeast (Ensembl 67)
Colorspace
Specify reference jar URL:
Check that reference jar URL exists...
Bowtie options
Don't forget to specify
-C
or
--color
if index is colorspace.
Model family
Poisson
Gaussian
Null permutations
 (If blank or 0, permutation test is disabled)
Gene Intervals
Intersection of transcripts
Union of exons
Options
Pool technical replicates
Pool all replicates
For paired-end reads, use just one mate
Options
Keep cluster running after job finishes/aborts
# EC2 instances
Instance type
c1.xlarge (recommended)
c1.medium
m2.xlarge
m2.2xlarge
m2.4xlarge
m1.xlarge
m1.large
m1.small
Made with the help of
Please cite
: Langmead B, Hansen K, Leek J.
Cloud-scale RNA-sequencing differential expression analysis with Myrna
.
Genome Biology
11:R83.